Shaxda tusmada
Biochemistry-ga, Lineweaver Burk Equation ee tamarta tamarta waxay keentaa Lineweaver Burk Plot oo sidoo kale loo yaqaan Plot laba-is-dhaafsi ah . Sidaa darteed, isticmaalayaashu waxay la yaabi karaan sida loo sameeyo Lineweaver Burk ee Excel.
Maqaalkan, waxaan ku muujineynaa habraac tallaabo tallaabo ah si loo sameeyo Lineweaver Burk plot in Excel.
Soo deji Buug-shaqeedka Excel >> >> Lineweaver Burk Plot.xlsx
Lineweaver Burk Plot iyo Its Qaybaha >>
Waa maxay Lineweaver Burk Plot?
A Sheekada waxaa loo istcmaalay in la is barbar dhigo iyada oo aan lahayn wax horjooge ah si loo aqoonsado waxtarka horjoogaha. Kuwan soo socdaa waxay qeexayaan qaybta Lineweaver Burk,
>2>, S . X- dhidibka khadka Lineweaver Burk kaas oo is-barbar-dhigaya diiradda substrate-ka, [ 1/S ].
Xawaaraha hore >
> Xawaaraha hore inta lagu jiro falcelinta enzyme-ka-hortagga ah, V ama V o . Y- dhidibka khadka lineweaver Burk ee isku celcelinta xawaaraha, [ 1/V o > ]. >xawaaraha ugu sarreeya >
Xawaaraha ugu sarreeya ee falcelinta enzyme-ka-hortagga ah, V max > . Dhexdhexaadinta dhidibka Y ee goobtu waa is barbar dhig xawaaraha ugu badan, [ 1/V max > ]. >
>MichaelisJoogtada
Michaelis Constant , K m > waa cabbirka xidhiidhka enzyme Dhexgalka dhidibka X ee goobta ayaa is barbar dhig u ah Michaelis Constant, [ -1/K m ].
Tallaabo-Tallaabo tallaabo ah a Lineweaver Burk Plot gudaha Excel
>> Samaynta Lineweaver Burk waxay u baahan tahay xogta Substrate Concentration ( S ) iyo Xawaaraha hore ( V o >). Isku celcelinta kuwa natiijadu waxay soo bandhigayaan Lineweaver Burk shirqool. >Mar tillaabooyinka soo socda si aad u samayso shaxda Lineweaver Burk gudaha Excel.Tallaabo 1: Dejinta Kor u qaad xogta >
> Isticmaalayaashu waxay u baahan yihiin inay ururiyaan xoogga substrate-ka ceeriin iyo xogta xawaaraha bilowga ah sida ku cad sawirka hoose. > > 3> 15> 16> Hel isdhaafsiga xogta cayriin ee labadaba (ie, S iyo V o >) sida lagu faray sawirka. > >>
> Tallaabada 2: > Gelida Shirqoolkii firdhinta
> > Ka dib dejinta xogta, isticmaalayaashu waxay u baahan yihiin inay geliyaan Shirqoolkii firdhinta . Sidii aan horay u soo sheegnay, fidinta qaabka firdhisanaanta xariiqda dib-u-dhaca waxay keenaysaa xayndaabka xargaha xargaha.- >
- Calaamad is-dhaafsiga, adigoo ka tagaya gelitaanka ugu horreeya ee aan qiimaha lahayn, ka dibna tag Geli > Geli firdhiso (gudaha Shaxda qaybta) > Guji Kala firdhi .
>
- Excel waxa ay gelisaa Scatter Plot sida ka muuqata sawirka dambe,isla markaaba
Akhrisyo la mid ah >
- >
- > Sida Loo Qorsheeyo Semi Log Graph gudaha Excel (oo leh Talaabooyinka Fudud) >
- >>Plot Sieve Sawirka Falanqaynta ee Excel (oo leh Tallaabooyin Degdeg ah)
Hadda, waxaan gelinay Scatter Plot anagoo adeegsanayna isdhaafsiga. Fidinta goobta kala firdhisan Trendline Backward natiijooyinka Lineweaver Burk plot.
> 15>>>>>> 22>>>> 3>
- >Excel waxay keenaysaa Format Trendline daaqada dhinaca. Daaqadda dhexdeeda,
- Marka hore, dooro Linear
- Dooro naqshad goob la door bidayo. Ugu dambeyntii, goobta Lineweaver Burk waxay u ekaan kartaa shaashadda hoose. >
Wax badan ka akhri Excel (Tallaabooyinka Fudud)
> >>> ⧭ Qoraallada: Lineweaver Burk Plot waxa laga yaabaa inay lahaato sawir ka duwan iyadoo ku xidhan xoogga qaybihiisa.Sidaa darteed, sawirka soo socdaa wuxuu soo bandhigayaa sawirro badan oo Lineweaver Burk ah oo kala saaraya xannibaado iyo falcelin la'aan. >
Maqaalku wuxuu ka hadlayaa hababka tallaabo-tallaabo ee samaynta Lineweaver Burk Plot ee Excel. Waxaan rajeyneynaa in maqaalkani uu iftiimiyo iftiin ku filan samaynta Lineweaver Burk si aad awood ugu yeelatid inaad mid sameyso.
Ka fiiri boggayaga cajiibka ah, Exceldemy, si aad u hesho maqaallo xiiso leh oo ku saabsan Excel